Worm Watch Lab Talk
Wow! Looks like a small bug under the plate. Could be the dreaded mite! http://bit.ly/1AhZxi8
Have to admit, I'm not sure either! A clump of food? Possibly an infection with another "non-food" bacterium?
Yes, and three eggs! Nice find.
The technical terms is "dumpy" 😃 http://www.wormbase.org/species/all/phenotype/WBPhenotype:0000583#0--10
Hard to tell indeed. Just do your best! (For this particular clip, I would go with no new eggs)
That's the tail 'whip' (high-res here: http://bit.ly/1MDcrXS). It's usually not resolved in these videos. Good find!
You're right, no eggs in this clip.
There are two eggs on the side of the worm, but they were already there at the start and so shouldn't be marked.
No fresh eggs there. Just the ones at the start.
That's for sure! Looks like she's been there a while...
Yes, the segmentation algorithm (that finds worm) is quite simple and has trouble with the food that sometimes piles up around the worm.
No egg in that video. But it is a cute slightly dumpy worm.
No egg laying there. Both eggs were present at the start from what I can see.
That's a patch of thick food the worm puts its head through. That can look like eggs on the side of the worm, but don't mark them.
You're right, no eggs in this one. The bulge around the worm at the start is the food it's crawling in.
No egg there. The sliver is a bit of food the worm moves.
That's right. Most segments won't have an egg laying event. If you watch a bunch, say 50, you should see some though.
The eggs on the side of the worm were laid earlier, so I wouldn't mark anything in this video.
Tough one, but I agree it's probably not an egg.
Some mutants are wider than others. Some are wide enough to qualify as "dumpy". Check out dpy-20, for example: http://bit.ly/1iPcwPp
Hmm, quite hard to say. Looks like two and two to me though.
Good eye. That's a larval worm stuck to the side of the adult. Check out the blog for a video of a worm laying a bunch at the same time.
This worm didn't lay an egg. Keep watching (maybe about 50 videos) and you'll see one eventually!
The worms are on the plates for about an hour so if you see more tracks that's just because they're more active.
I would go with food pile for that one.
Sometimes if the outline is rough, the worm shape isn't properly detected and we don't draw the outline.
looks alive and kicking to me!
I only see one egg in this video (at around 0:02).
That one's quite hard to see, but I think you're right that there are no new eggs.
We're still on the first 75 000 clips so nothing has been removed yet. We'll be taking a first look at the data soon and will update then.
It looks like only two new eggs. There was one egg already there that the worm crawled into.
I agree, it does look like an egg. It's also possible that it's a vulval defect. It's difficult to distinguish at this resolution...
Yes! Make sure you hit z twice so we know there were two!
That's true, but please only mark the eggs laid in the current clip. In this case there's one egg laid right at the start.
I think I can still see a little bit of motion at the tip of the head. It might just be quiescent or severely uncoordinated.
Looks like two around the same time to me.
They were laid before the start and the worm crawled into them. Might be hard to tell because they're there right at the start.
There are no eggs in this one. The worm is at the edge of the food patch where it's thick and sometimes the food piles look like eggs.
It is an egg, but don't mark this one because it was laid before the start of the video.
No, just uncoordinated!
For some mutants that retain their eggs for a long time it can happen regularly. For normal worms on food I wouldn't expect to see it.
That's a good one. The worm laid larvae and they start to crawl away! That's why you can't see eggs at the end of the video.
The video looks ok to me. It must not have loaded properly when you were trying to watch it.
Good eye, but those aren't eggs. That's actually a larval worm crawling around on its mother!
Most of the videos won't have any eggs being laid. You will need to get through 30 or 40 to find one with eggs. Thanks for your help!
Normal worms are sometimes active and sometimes inactive. Some mutants are active most of the time and/or are faster.
Nematodes move by bending their bodies. They move more like snakes than like earth worms.
That looks like the edge of the plate. Sometimes the tracker gets confused and loses the worm at the edge of the plate.
That can happen in worms that retain their eggs for a long time (so long that they hatch inside the mother!).
Yes, the program looks for a shape with a relatively smooth outline so if there are lots of peaks caused by the food it gets confused.
I agree. I can't quite decide if it's just a wetter than normal plate or if it's a problem with the worm. Probably both...
Actually not sure... Maybe somehow some food got stuck. Some mutants have vulval development defects, but I don't think that's it.
Yes, those are previously laid so don't mark those.
It should be right most of the time, but sometimes we get the side wrong. It's good to know though, so thanks for pointing it out.
In thick food, our algorithm isn't very good at finding the worm edge so it sometimes won't draw the outline.
I think it's most likely a bubble.
The green dot is on the tail. #wrongheaded
This worm doesn't seem to be getting anywhere! #treadmill
That's right. That one was there from before the start of the video. Don't mark that one.
Our analysis program can't currently determine the midline when the worm loops so it doesn't draw anything in those cases.
It is!
Yes, the tracking algorithm is quite simple (it just follows the largest object), so if the worm reaches the edge it's lost.
Don't mark those ones, only the ones that it lays during the current video.
Thanks for pointing this out. That's useful information for us.
If the worm edge isn't clear, our algorithm can't accurately identify it so it doesn't draw the outline. But you can sitll look for eggs!
That's right, don't mark that one.
If you think you might have seen an egg, you can pause the video by clicking on it. Then you can scroll to find exactly when it was laid.
I wouldn't mark any in that case. The eggs will always come out the side of the worm around the middle, but not at the tail.
Excellent 😃
That's a tough one. In that case I wouldn't mark an event unless you could clearly see one added to the pile.
Yes, there is! That one's already hatched. Good eye.
That's right, but don't mark those in this video, only ones that you see pop out of the side of the worm!
Yes, those are eggs, but don't mark them in this video because they were laid before the start of the video.
Yes, those are eggs (two below and one above the worm). But remember not to mark those since they were laid before the start of the video.
I don't see one in this video, but in rare cases an egg-laying defective worm can retain eggs so long that they hatch inside the mother.
It's not actually released by the worms, it's a track they leave in their food. (the plate is covered with bacteria that they eat)
Good catch! It's probably a 1st larval stage worm since the adults are only on the plate for a short time.
I don't think it's dead (well, not yet), just a very uncoordinated mutant. If you look closely you can see it sometimes twitches its head.
This worm leaves the patch of food and crawls outside the range of the tracker's stage. #greatescape
This worm seems to be slipping on the surface and not making much progress despite the effort. #treadmill
#wrongheaded
Have faith, at least some of the videos show egg laying! 😃 This one at 15.9 seconds.
This worm has the green dot on its tail. #wrongheaded
This worm is incorrectly annotated. The green dot is actually on the tail in this video. #wrongheaded
Wow! Looks like a small bug under the plate. Could be the dreaded mite! http://bit.ly/1AhZxi8
Have to admit, I'm not sure either! A clump of food? Possibly an infection with another "non-food" bacterium?
Yes, and three eggs! Nice find.
The technical terms is "dumpy" 😃 http://www.wormbase.org/species/all/phenotype/WBPhenotype:0000583#0--10
Hard to tell indeed. Just do your best! (For this particular clip, I would go with no new eggs)
That's the tail 'whip' (high-res here: http://bit.ly/1MDcrXS). It's usually not resolved in these videos. Good find!
You're right, no eggs in this clip.
There are two eggs on the side of the worm, but they were already there at the start and so shouldn't be marked.
No fresh eggs there. Just the ones at the start.
That's for sure! Looks like she's been there a while...
Yes, the segmentation algorithm (that finds worm) is quite simple and has trouble with the food that sometimes piles up around the worm.
No egg in that video. But it is a cute slightly dumpy worm.
No egg laying there. Both eggs were present at the start from what I can see.
That's a patch of thick food the worm puts its head through. That can look like eggs on the side of the worm, but don't mark them.
You're right, no eggs in this one. The bulge around the worm at the start is the food it's crawling in.
No egg there. The sliver is a bit of food the worm moves.
That's right. Most segments won't have an egg laying event. If you watch a bunch, say 50, you should see some though.
The eggs on the side of the worm were laid earlier, so I wouldn't mark anything in this video.
Tough one, but I agree it's probably not an egg.
Some mutants are wider than others. Some are wide enough to qualify as "dumpy". Check out dpy-20, for example: http://bit.ly/1iPcwPp
Hmm, quite hard to say. Looks like two and two to me though.
Good eye. That's a larval worm stuck to the side of the adult. Check out the blog for a video of a worm laying a bunch at the same time.
This worm didn't lay an egg. Keep watching (maybe about 50 videos) and you'll see one eventually!
The worms are on the plates for about an hour so if you see more tracks that's just because they're more active.
I would go with food pile for that one.
Sometimes if the outline is rough, the worm shape isn't properly detected and we don't draw the outline.
looks alive and kicking to me!
I only see one egg in this video (at around 0:02).
That one's quite hard to see, but I think you're right that there are no new eggs.
We're still on the first 75 000 clips so nothing has been removed yet. We'll be taking a first look at the data soon and will update then.
It looks like only two new eggs. There was one egg already there that the worm crawled into.
I agree, it does look like an egg. It's also possible that it's a vulval defect. It's difficult to distinguish at this resolution...
Yes! Make sure you hit z twice so we know there were two!
That's true, but please only mark the eggs laid in the current clip. In this case there's one egg laid right at the start.
I think I can still see a little bit of motion at the tip of the head. It might just be quiescent or severely uncoordinated.
Looks like two around the same time to me.
They were laid before the start and the worm crawled into them. Might be hard to tell because they're there right at the start.
There are no eggs in this one. The worm is at the edge of the food patch where it's thick and sometimes the food piles look like eggs.
It is an egg, but don't mark this one because it was laid before the start of the video.
No, just uncoordinated!
For some mutants that retain their eggs for a long time it can happen regularly. For normal worms on food I wouldn't expect to see it.
That's a good one. The worm laid larvae and they start to crawl away! That's why you can't see eggs at the end of the video.
The video looks ok to me. It must not have loaded properly when you were trying to watch it.
Good eye, but those aren't eggs. That's actually a larval worm crawling around on its mother!
Most of the videos won't have any eggs being laid. You will need to get through 30 or 40 to find one with eggs. Thanks for your help!
Normal worms are sometimes active and sometimes inactive. Some mutants are active most of the time and/or are faster.
Nematodes move by bending their bodies. They move more like snakes than like earth worms.
That looks like the edge of the plate. Sometimes the tracker gets confused and loses the worm at the edge of the plate.
That can happen in worms that retain their eggs for a long time (so long that they hatch inside the mother!).
Yes, the program looks for a shape with a relatively smooth outline so if there are lots of peaks caused by the food it gets confused.
I agree. I can't quite decide if it's just a wetter than normal plate or if it's a problem with the worm. Probably both...
Actually not sure... Maybe somehow some food got stuck. Some mutants have vulval development defects, but I don't think that's it.
Yes, those are previously laid so don't mark those.
It should be right most of the time, but sometimes we get the side wrong. It's good to know though, so thanks for pointing it out.
In thick food, our algorithm isn't very good at finding the worm edge so it sometimes won't draw the outline.
I think it's most likely a bubble.
The green dot is on the tail. #wrongheaded
This worm doesn't seem to be getting anywhere! #treadmill
That's right. That one was there from before the start of the video. Don't mark that one.
Our analysis program can't currently determine the midline when the worm loops so it doesn't draw anything in those cases.
It is!
Yes, the tracking algorithm is quite simple (it just follows the largest object), so if the worm reaches the edge it's lost.
Don't mark those ones, only the ones that it lays during the current video.
Thanks for pointing this out. That's useful information for us.
If the worm edge isn't clear, our algorithm can't accurately identify it so it doesn't draw the outline. But you can sitll look for eggs!
That's right, don't mark that one.
If you think you might have seen an egg, you can pause the video by clicking on it. Then you can scroll to find exactly when it was laid.
I wouldn't mark any in that case. The eggs will always come out the side of the worm around the middle, but not at the tail.
Excellent 😃
That's a tough one. In that case I wouldn't mark an event unless you could clearly see one added to the pile.
Yes, there is! That one's already hatched. Good eye.
That's right, but don't mark those in this video, only ones that you see pop out of the side of the worm!
Yes, those are eggs, but don't mark them in this video because they were laid before the start of the video.
Yes, those are eggs (two below and one above the worm). But remember not to mark those since they were laid before the start of the video.
I don't see one in this video, but in rare cases an egg-laying defective worm can retain eggs so long that they hatch inside the mother.
It's not actually released by the worms, it's a track they leave in their food. (the plate is covered with bacteria that they eat)
Good catch! It's probably a 1st larval stage worm since the adults are only on the plate for a short time.
I don't think it's dead (well, not yet), just a very uncoordinated mutant. If you look closely you can see it sometimes twitches its head.
This worm leaves the patch of food and crawls outside the range of the tracker's stage. #greatescape
This worm seems to be slipping on the surface and not making much progress despite the effort. #treadmill
#wrongheaded
Have faith, at least some of the videos show egg laying! 😃 This one at 15.9 seconds.
#wrongheaded
#wrongheaded
This worm has the green dot on its tail. #wrongheaded
This worm is incorrectly annotated. The green dot is actually on the tail in this video. #wrongheaded